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Lightning Talks are concise 3-5-minute oral presentations that tell the big picture story of your research. A good rule of thumb for an oral presentation is that it takes about a minute to discuss a slide, so your talk should have no more than ~5 slides.
This is very short time, so focus on the big questions that guided your research, rather than details of what you did. Think about addressing things like what motivated your project, what did you do to answer your research question, what answers did you find and why it all matters.
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